Industrial quality control
Due to miniaturisation and mass customization, the demands on quality control have increased heavily. Quality control in many industrial fields can benefit greatly from the Dino-Lite digital microscopes. Checking the quality of milling, painting, assembly, manufacturing, tooling has already become daily work for Dino-Lite in thousands of industrial companies. Dino-Lite is easy to use and affordable, but also offers digital storage of images and video's with software options such as measurement, annotation and comparing images.
Highly innovate industries such as automotive, aeronautics, electronics, medical device manufacturers can greatly benefit from Dino-Lite because of the speed and portability of both the regular USB Dino-Lite models, the high speed models or even the Dino-Lite mobile solution with 5 inch portable screen. More traditional industries such as print and paper, paint and lacquer, metal and plastics production, textiles but also foodstuffs, packaging and many more can now use microscopy across the full production chain. In all industries, one can now easily exchange quality information with suppliers and customers but also assure high level product support and problem solving across the supply chain.
Dino-Lite digital microscopes can be implemented in larger industrial systems and integrated into existing software solutions with the Software Development Kit (SDK). Or you can digitize existing microscopy equipment with one of the ocular camera's from the DinoEye eyepiece range that are is software compatible with Dino-Lite. Software partners have made application solutions for specific industries, please check the software menu on this website.
Evaluating enzyme activity on wood pulp
The Dino-Lite digital microscopes are used for many tasks. The French company Celodev is using the metal Dino-Lite Pro X models to evaluate enzyme activity on wood pulp. Celodev is the producer of enzymes used in the paper industry, primarily to reduce the energy consumption for paper production. This requires an explanation: wood pulp is the raw material of paper, it is a slurry of small particles that are made from wood fibers. Certain enzymes such as cellulase, hemi-cellulase, pectinase and β-glucanase are able to break down these fibers and make the pulp suitable as a raw material for making paper. This biodegradation is used as an alternative to the energy-consuming mechanical refining of the fibers. Resulting in a lot of electricity saved, with great environmental benefit.
Gilles Bajul, the owner of Celodev: "The enzymes which we make break down the pulp, and thus provide for a partial and controlled refining of the fibers. The enzymatic treatment of the pulp reduces the energy consumption of the paper production, and it also improves the properties of the paper. We have to validate the enzymes that we provide constantly. In our laboratory we assess whether the various enzymes do their work in the right way, by reviewing the decomposition process under the Dino-Lite microscope. The nice thing about the Dino-Lite digital microscope is that it is robust and we can arrange the amount of light easily. This means that we do not need other light sources. The size makes the Dino-Lite a very convenient device. We take pictures but also movies with the Dino-Lite and we can store and process this material immediately because everything is digital and this saves us a lot of time. Dino-Lite has made itself indispensable in our lab."
Dino-Lite in primary education
The Dino-Lite USB microscope gives students instant access to the small world. Up to 200 times magnifications give a wonderful insight to details that normally stay hidden.
Just connect the Dino-Lite Basic (AM2111) to the PC and you get instant results on the screen. You can study insects, electronics, printed materials and so much more. The Dino-Lite Basic has a price performance that makes it suitable for use by individual students in the classroom. The Dino-Lite Pro is the ideal solution to show and discuss microscopic images in the classroom. Use the Dino-Lite Pro (for instance AM4113T or AM7013MT) with its high resolution on a digital blackboard or on a screen.
This Dino-Lite model features a touch button to capture images directly. With the DinoCapture software you can even make measurements on the pictures. To make really good pictures with the Dino-Lite, the use of a stand is recommended. The flexible stand (MS33W) can be oriented in all directions. The professional stand MS35B offers even more precision. The position of the Dino-Lite can be fixed and adapted vertically.
Dino-Lite shows micro-electronics to students
The 'Science-on-Tour' travelling show wants to grasp the interest for technology of pupils between 10 and 18 and encourage them to develop their talents in the field of engineering and science. The travelling show was initiated by the University of Twente (the Netherlands) and is a collection of active experiments to watch and do. They are based on surprising and intriguing physical and chemical phenomena. Thomas Hoen is one of the students participating in the Tour as part of the electro technical team. He is a second year student of Electrical Engineering. Apart from his team, there is a team for chemistry and a physics team involved in 'Science on Tour'.
"We show other pupils how small electrical moving parts can be", Hoen explains. "We have mounted moving parts on a chip measuring two millimeters. They are so small that you can hardly perceive any movement with the naked eye. We have placed a Dino-Lite Pro on a tripod above the chip. The Dino-Lite is connected to a laptop by USB and the students can clearly see the movement on the screen of the laptop. The Dino-Lite with a five megapixel resolution shows the object with a magnification of 200 times. This is obviously much easier than a microscope where students take turns to stare. The built-in LED makes the setup very easy since we do not need to install a separate light source.
Everyone finds it fascinating to see how you can install moving part on such a small surface. Especially when I tell that someone has managed to build in a working steam engine on a one by one mm surface. Unfortunately we not show this! All in all, I hope that we can interest students in technical education with these beautiful sharp images and that we can literally show that technical education is not dull at all, but very exciting. Dino-Lite helps us do that!
Dino-Lite in science education
A Dino-Lite Digital microscope is a versatile piece of equipment, and one of its many applications is Education. A great example of this can be found in the unique Maritime Bus owned by The Hampshire & Wight Trust for Maritime Archaeology, a registered charity. In the bus a special 'Microscope Corner' can be found, providing all visitors with a close up look at Maritime archaeological artefacts and materials.
Amanda Bowens, Education & Outreach Manager of the HWTMA explains: "The Hampshire & Wight Trust for Maritime Archaeology have been using the Dino-Lite AM4112PT Digital Microscope in our Maritime Bus, which offers free public outreach sessions for members of the public and community groups, for several years. It's the perfect tool for people of all ages and backgrounds to have a hands-on experience, and study the microscopic detail of archaeological artefacts and materials in the Bus. The microscope corner on the Maritime Bus is extremely popular, we feel this is because it offers a fun and interactive way to learn a great deal about our sunken heritage. Having looked at a range of options, the Dino-Lite was the only microscope that could do the job for us."
Recognizing product falsifications with Dino-Lite
Counterfeiting or falsifying products is a global problem that makes many manufacturers suffer. It is not only common for food and non-food, soft drinks, perfumes and pharmaceutical products to the fast moving consumer goods and the automotive industry. Counterfeiting is not just an Asian issue, Europeans can also play this game. It is estimated that more than ten percent of all products are counterfeits, which constitutes a global loss between 200 and 300 billion euro each year. Enough reason to take serious efforts to protect your products against counterfeiting. A leading security company in France works with manufacturers with the help of Dino-Lite digital microscopes and proprietary software that was adapted with the Dino-Lite Software Development Kit (SDK).
This process allows manufacturers to apply an authenticity feature to their products. Their product manager explains: "This can be a code with a dot matrix, but it can also be engraved or even stuck in the cap of a product. This code tells us everything about the product and allows us to see if it's a real product, a forgery or a so-called gray product, the origin of which is not immediately obvious. You can imagine that counterfeit drugs are dangerous and can even be lethal. More than enough reason for the creation of the Anti-Counterfeiting Trade Agreement, known as ACTA.
"We are using Dino-Lite to read and recognize the codes with our own software. With the Software Development Kit (SDK), supplied by Dino-Lite, we have adjusted our own software to work with Dino-Lite and all its features. On behalf of our clients we do worldwide research into product authenticity. Our people go in the field with a Dino-Lite, linked to a laptop where that runs our software. By 'scanning' the product, we can quickly determine whether something is genuine or counterfeit. Based on our findings, the producer can then take action, as counterfeiting is still a criminal offense."
At present this company uses approximately thirty Dino-Lites. But it is expected that this number will increase rapidly because of the ease of use and the modest size of the Dino-Lite.
Faster diagnosis scleroderma with Dino-Lite CapillaryScope
If a patient has suspected systemic sclerosis, the Dino-Lite CapillaryScope can be used to make a quick and painless examination of the capillaries in the cuticle. Actelion Pharmaceuticals Ltd cooperates with rheumatologists to make this a generally used procedure quickly.
Actelion Pharmaceuticals Ltd is originally a Swiss biopharmaceutical company focused on the discovery, development and marketing of orphan drugs on the market. "These are medications for unresolved medical problems which in most cases involves a small group of patients," says brand manager Peter Dingeldein of the Dutch division of Actelion Pharmaceuticals Ltd. Systemic sclerosis is one of the rare diseases for which the company is providing a drug. This rare chronic disease is caused by a disordered immune system, which is why this is called an autoimmune disease. In systemic sclerosis healthy tissue gets damaged and creates connective tissue. The vasculopathy is an important characteristic of this disease, that often results in digital ulcers, painful sores on the fingers. Once patients arrive at this stage, this will have major impact on everyday life, as everyday activities, such as buttoning clothes and writing difficult.
Dingeldein: "There is increased attention to discover systemic sclerosis as early as possible. The idea is that the sooner you discover the disease, the better it is for the patient because the damage can be limited. If a patient is suspected of systemic sclerosis, then studying the capillaries in the cuticle can provide more information about the diagnosis. This study goes well and fast with a Dino-Lite CapillaryScope. It is a simple, non- invasive and painless test that patients can watch themselves. That is reassuring for many people."
For the examination a drop of oil is placed on the cuticle and the CapillaryScope is placed on that spot. The capillaries in the cuticle are clearly visible with the magnification of 500x and thus any abnormalities suggestive of systemic sclerosis can be examined.
In most cases, the rheumatologist can reassure the patient that there is nothing to worry about. For anomalies it is easy to make a picture or recording with the CapillaryScope to save to the patient's file. At the repeat visit, usually after six months, it can be established whether the situation has improved, remained stable or deteriorated. When a patientis diagnosed with systemic sclerosis the treatment – medication - can begin immediately.
Endothelin is the enzyme in the body that constricts blood vessels. In diseases such as systemic sclerosis, an excessive amount of endothelinis produced that ultimately create damage to the vessel walls. Actelion Pharmaceuticals Ltd is a manufacturer of Tracleer ( the generic name is Bosentanname , ed.). This is a vasodilator drug that inhibits the action of endothelin by blocking the nerve receptors that can bind endotheline which will improve the blood flow. Tracleer does not heal, but prevents worse and helps to stabilize the patient.
This is capillaroscopy:
The investigation of the cuticle with the Dino-Lite CapillaryScope is quick and painless. Click on the link to see a movie, that shows exactly how the research process works.
Cell research made visible with
USB fluorescence microscopy
Dino-Lite helps researchers to form an image
Research into life-threatening diseases is of great importance. Miraculously a small striped fish with special light microscopy can play an important role. Professor Yung-Jen Chuang (47) from Taiwan is doing research with zebrafish using Dino-Lite fluorescence microscopes.
Within the National Tsing Hua University in Hsinchu, Taiwan, Professor Yung-Jen Chuang runs a laboratory for vascular biology. Vascular biology is the study of our circulatory system in all its forms, from the aorta to the smallest capillary in the brains. Professor Yung-Jen Chuang and his team are particularly interested in the molecular and cellular processes that occur when new blood vessels are formed from the existing blood vessels, a process that is called angiogenesis. The team is also investigating how tissue repair occurs after injury to vital organs such as heart or brains, and examines which reactions influence the blood circulation within a tumor. The studies also involve functional genomics that aims to identify what specific genes work harder, for instance to speed up regeneration. Obviously Professor Yung-Jen Chuang is leading a team that consists of a large number of researchers, an even greater number of zebrafish and Dino-Lite fluorescence microscopes.
Excitation and emission
Dino-Lite has different fluorescence microscopes that are used by Professor Yung-Jen Chuang in his investigations. Fluorescence microscopy makes use of fluorescent dyes that give off light when it is irradiated with light of a shorter wavelength. This light, for example blue, is referred to as excitation light. The phosphors convert this light of a short wavelength into light of a longer wavelength, such as green or red. This emitted light is called emission light. The Dino-Lite fluorescence microscopes have a so-called excitation filter that limits the outgoing light to a certain wavelength (expressed in nanometers). In the same way, the emission light is going through a built-in emission filter that removes the excitation wavelength from the light bundle and the image only consists of the emission light. In this way, Professor Yung-Jen Chuang can see what happens in a living organism in real time. One of the animals he is using for this purpose is the zebrafish. Since the 90s of the last century many scientists are using zebrafish (scientific name Danio rerio) as a model organism. The genome (the total of genetic information in a cell) of a zebrafish is very similar to the genome of human beings. Zebrafish embryos are also translucent, making it possible to study various processes such as angiogenesis in an organism using fluorescence microscopy. The zebrafish is not naturally fluorescent, so how were scientists able to give the fish light?
GFP, BFP, CFP, YFP and CherryFP
This became possible after Nobel laureate Osamu Shimomura discovered that the deep sea jellyfish Aequoria was naturally fluorescent and owes this property to a protein called green fluorescent protein (GFP). This protein can be compared to a beacon that brightened up biological research. In 1994 scientists were able to transfer this gene into the cells of higher organisms through transfection, the technique in which foreign DNA is placed into a cell. It was now possible to highlight each cell in color using fluorescence microscopy, which was a revolution in cell biology. But the scientists went further. By causing mutations in the GFP-gene, they were able to change the color of the fluorescence. Now there is not only green (GFP), but also blue (BFP), cyan (CFP), yellow (YFP) and red (CherryFP). Both the excitation and emission spectra of these variants differ so that different models Dino-Lite were made to observe the different colors. Most fluorescence microscopes have a traditional emission filter whereas Dino-Lite uses high-pass filters that provide enhanced imaging and improved sensitivity in a wider spectrum of the fluorescence.
The GFP color variants give researchers the ability to let for instance arteries emit green light, immune cells red and bacteria blue. In this way an investigator can follow the occurrence and progression of a disease, such as cancer. In order to investigate the conduct of a cancer type, human cancer cells labelled with fluorescence are implanted into zebrafish embryos. This makes it possible to monitor all stages of the development of a cancer and allows research into the genes and substances involved in the development of cancer and the inhibition of the growth of the tumor cells. Professor Yung-Jen Chuang's team is obviously not the only one doing this type of research. Thousands of researchers all over the world are working with zebrafish and fluorescence microscopy to study diverse diseases like Parkinson's, multiple sclerosis and acute lymphocytic leukemia hoping to find solutions. In many cases, Dino-Lite literally helps researchers to form a good picture of the disease and healing processes that occur in an organism. With this knowledge drugs can be developed to inhibit, heal and even prevent diseases like cancer.
Professor Yung-Jen Chuang worked with Dino-Lite to develop the fluorescence digital microscopes: "I am delighted that the Dino-Lite fluorescence microscopes are of good quality and affordable. Moreover, they are easy to use. Thus, we can enable more researchers to work after minimal training, and also enlist various sets of Dino-Lites that we have for educational purposes. It is easy to show the images on a laptop, and we can store both video and still images to study changes in tissue better." Currently, Yung-Jen Chuang has achieved most successes in studying the interaction between a host such as a human and a fungal pathogen such as Candida albicans.
What Dino-Lite models does Professor Yung-Jen Chuang use?
In his laboratory in Taiwan Professor Yung-Jen Chuang uses three types of fluorescence microscope and a general Dino-Lite. The Dino-Lite fluorescence microscopes are the smallest fluorescence microscopes in the world. The user can switch from colored LEDs to the built-in white LED, which is useful for focusing and locating the object.
The Dino-Lite AM4115T-GFBW is a fluorescence microscope equipped with blue LEDs. The emission filter of 510 nanometers ensures that Green Fluorescent Protein (GFP) shows up.
The Dino-Lite AM4115T-RFYW uses yellow LEDs and an emission filter of 610 nm. This microscope is very useful in developmental biology, pathology and anatomy.
The Dino-Lite AM4115T-YFGW features green LEDs for excitation. The 570 nm emission filter of this fluorescence microscope ensures that orange to red fluorescence such as RFP (Red Fluorescent Protein) shows up.
AM4113ZT is not a fluorescence microscope, but is also used in the lab of Professor Yung-Jen Chuang. This is one of the top selling Dino-Lite models. This USB microscope is provided with a rotatable polarizing filter so that research on shiny objects becomes simpler, because disturbing reflections are reduced significantly.
Fluorescence Guided Surgery, FGS.
For surgeons it is of the utmost importance to be able to able to see accurately the tumour margins during the surgery. This is of particular importance for resection of metastatic diseases. An important and For FGS, clinically approved fluorescent dyes are being used to label the abnormal cells or diseased tissue. Illuminating the area of interest with the corresponding excitation light and the use of a correct emission filter in front of the imaging device, the cancerous tissue will light up and can be removed easily and accurately. With this method smaller tumours can be removed, opposed to the standard methods of visualisation and detection. FGS is nowadays considered as an effective way to carry out cancer surgery in comparison to the conventional surgery. Researchers around the world are exploring the possibilities of this emerging technology.
In the following 4 open access research papers, several different Dino-Lite Fluorescence microscopes have been used to perform Fluorescence Guided Surgery successfully.
FGS of Liver Metastasis in Orthotopic Nude-Mouse Models:The fluorescence Dino-Lite model AM4113T-GFBW has been used to visualize the green fluorescence protein (GFP) labelled tumour before, during and after the surgery.
The researchers mentioned below have performed a trial to show prove that FGS is an effective method for liver metastasis. Fourteen 14 mice of the created orthotopic liver metastasis model had been randomly divided in 2 groups. One group was treated with BLS (Bright Light Surgery) and the other group was treated with FGS by using the Dino-Lite fluorescence digital microscope. Post-surgical residual was clearly present with the BLS treated mice, while the FGS group showed no sign of residual tumor.
Paper: Murakami T, Hiroshima Y, Zhang Y, Chishima T, Tanaka K, Bouvet M, et al (2015) Fluorescence-Guided Surgery of Liver Metastasis in Orthotopic Nude-Mouse Models.
Link: PloS ONE 10(10): e0138752. Doi:10.1371/journal.pone.0138752
FGS using In Situ GFP Labeling with a Telomerase-Dependent Adenovirus in an Orthotopic Mouse Model
In this research article they demonstrate that labelling colon-cancer liver metastasis in-situ with the GFP OBP-401 adenovirus is a powerful tool to complete resection with FGS. Compared to the conventional BLS ( Bright Light Surgery) it reduced the recurrence rate and prolonged the over-all survival. FGS was performed under GFP guidance using amongst others the Dino-Lite fluorescence microscope AM4113T-GFBW.
Paper: Yano S, Takehara K, Miwa S, Kishimoto H, Hiroshima Y, Murakami T, et al. (2016) Improved Resection and Outcome of Colon-Cancer Liver Metastasis with Fluorescence-Guided Surgery Using In Situ GFP Labeling with a Telomerase-Dependent Adenovirus in an Orthotopic Mouse Model.
Link: PLoS ONE 11(2): e0148760. doi:10.1371/journal. pone.0148760
Color-coded FGS to Resect the Tumor along with the Tumor Microenvironment“Color coded FGS is an effective method to completely resect cancer cells along with the stromal cells in the TME which interact in a highly-complex pattern.“
Results: Using the Dino-Lite, subcutaneous tumors and the tumor microenvironment were clearly visualized and resected.” During this research they have used the AM4113T-YFGW to visualize the EL-4-RFP stained cells and the AM4113T-GFBW for the GFP stained cells.
Paper: Color-coded Imaging Enables Fluorescence-Guided Surgery to Resect the Tumor Along with the Tumor Microenvironment in a Syngeneic Mouse Model of EL-4 Lymphoma. Hasegawa K, Suetsugu A, Nakamura M, Matsumoto T, Kunisada T, Shimizu M, Saji S, Moriwaki H, Bouvet M, and Hoffman R.
Link: Anticancer research 36: 4443-4448 (2016). doi:10.21873/anticanres.10988
Color-coding cancer and stromal cells with genetic reporters enhances FGSAlso here GFP containing the OBP401 was used to label the cancer cells of the pancreatic cancer PDOX. The PDOX was previously grown in a RFP transgenic mouse. This dual color-coding enabled FGS to completely resect the pancreatic tumors including stroma. “Dual-colored FGS significantly prevented local recurrence, while bright-light surgery (BLS) or single color could not”. “The Dino-Lite mobile imaging system was used for imaging in live mice.” “This all-in-one compact digital camera makes the Dino-Lite imaging system easily transportable and thereby suitable for FGS”.
Paper: S Yano, Y Hiroshima, A Maawy, H Kishimoto, A Suetsugu, S Miwa, M Toneri, M Yamamoto, M H G Katz, J B Fleming, Y Urata, H Tazawa, S Kagawa, M Bouvet, T Fujiwara and R M Hoffman. Color-coding cancer and stromal cells with genetic reporters in a patient-derived orthotopic xenograft (PDOX) model of pancreatic cancer enhances fluorescence-guided surgery.
Link: Cancer Gene Therapy 22, 344-350 (July 2015) | doi:10.1038/cgt.2015.26
Dino-Lite supports higher education in Africa
Tom Baden is a postdoctoral neuroscientist at the University of Tübingen in Southern Germany. His research is mainly focused on the eyesight of mice. Together with his colleague Lucia Prieto of Lausanne University, Switzerland, he recently founded the "TReND in Africa" volunteer organization. The acronym stands for Teaching and Research in Neuroscience for Development. Sadiq Yusuf of Kampala International University, Uganda, was also involved in the founding of TReND in Africa. Dino-Lite has donated several digital microscopes to the organization. Tom: "We are very happy with this as we completely rely on gifts and used lab material.
TReND in Africa aims to acquaint upcoming scientists from African countries with neuroscience and neurogenetics. "This is the third year that we have organized a summer school", says Tom Baden. "We teach students from all over Africa on site in neurosciences and neurogenetics. Traditionally, much research in the area is being carried out using mice and rats, but these are quite expensive as test animals. Therefore we teach our students to work with fruit flies and other insects that are much cheaper and well suited to serve as model systems a wide range of neuroscientific questions."
The Dino-Lite is very suitable to study the transgenic fruit flies. "In combination with the right filters, the Dino-Lite with UV light can work to visualize genetically encoded fluorescent markers in both the fruit flies and their larvae. And we use them when dissecting larger invertebrates such as crickets and cockroaches to teach the students the basics of electrophysiological techniques. The white light of the built-in LED's is very helpful for this. The Dino-Lites are easy to use and have a very high image quality. We look at the images on the screens of the computers in the lab."
At the end of the summer course Baden left the Dino-Lites behind. Tom: "Our target is not only transferring knowledge but also helping to increase the level of the higher education in Africa. By bringing knowledge and leaving teaching materials behind we hope that university students can reach a higher level and the number of African scientists will increase."
Textile Technology and education with Dino-Lite
John McLoughlin is Senior Lecturer at Manchester Metropolitan University in England. He is teaching 'Fashion and textile technology' and is considered to be an expert when it comes to fabrics, fabric processing and joining techniques. He has published extensively on the subject. McLoughlin is using a Dino-Lite digital microscope as an integral part of his lectures, to show magnified images of cloths and stiches. McLoughlin: "I project an image on screen so that all students can see the materials in detail. I do this live in all my lectures and also let students work with the Dino-Lite themselves, so that they gain experience with it."
McLoughlin is very satisfied with the performance of the Dino-Lite." I used to work with another brand that was more expensive and the picture quality was much lower. The Dino-Lite is also very easy to use, you just connect the USB cable and it is ready to go. I would love to see each student havingtheir own Dino-Lite and I am a strong advocate for this.
McLoughlin is now developing software that can recognize the physical characteristics of a cloth and determine how it should be labored. This involves issues like: what type of stitch, which density and what needles are most suitable for the job. McLoughlin: "I have also used the Dino-lite to collect data for my software. Without the Dino-Lite I could not have developed the software. This software will prove itself especially in the mass production of textiles. It will allow further automation of the production process."
McLoughlin is convinced that Dino-Lite helps to deliver students that are much better equipped for their future job: "A handy microscope is indispensable to grasp the essence of my lectures. You need this level of detail to really know what you're talking about. If I say that a fabric is hairy, I want to show at the fiber level why I call the substance hairy. Of course it would be ideal if every student can just go home and check out fabrics with a Dino-Lite. Dino-Lite brings my topic to life."