ideal for restoration of
ethnographic and art objects
Dino-Lite digital microscopes can be a solution for the restoration of archaeological and art objects. This mobile microscope is a useful tool because it makes details visible and is flexible in application. Restorers of the Tropenmuseum recently experienced this. This year the Tropenmuseum in Amsterdam has restored ten bisj poles. Thanks to the Dino-Lites, the museum visitors were able to observe the achievements of the conservators down to the wood fiber level. The Dino-Lite also proved to be a valuable tool for restoring down to the square time-lapse millimeter.
Reverence for the dead
With a height of up to ten meters, the bisj poles were made in the last century by the Asmat, a Papuan people in former Dutch New Guinea. Using these richly decorated nutmeg wood poles, the dead are still being honored. The bisj pole ensures that the soul of the deceased can ascend to the afterlife.
The Tropenmuseum has twelve poles of this kind from the last century. Ten have been restored. Extremely careful cleaning was the most important task. The restorers cleaned every inch of the poles using a soft brush, also called a dry restoration sponge, that was specifically chosen for cleaning these objects. Not damaging the original paint layers was the main goal. The poles are painted with natural pigments, like burnt ochre and ground shells. These are vulnerable materials. They used a brush to clean the wood. They therefore barely needed to touch the wood, and the dirt was removed.
Precision work was required. The human eye cannot discern these subtle nuances for long. One restorer said: “Using the Dino-Lite microscope, we easily noticed the difference between dirt, overpainting and the original paint layer. We used this microscope before and after cleaning the surface. That was the only way to easily notice the changes. In this area, the Dino-Lite was also very flexible.” The time-lapse function was also very handy during the recovery process. It could be used to document how different layers were applied onto the original paint layer.
Viewing the work on a screen
The restoration took place in the Lichthal, the central hall of the museum. “In this way, the visitors could easily observe our work. An extra dimension was added because visitors could watch on the screen every detail of what we saw” A museum visitor can’t get closer to a restoration process.
For the first time, the restorers of the Tropenmuseum were introduced to the possibilities of the Dino-Lites. They liked to use them, because the enlargements of details and the time-lapse function have contributed to the quality of the restoration work. Because of these experiences, the museum decided that the Dino-Lites can also be used in future restorations. The successful restoration project in the Tropenmuseum showed that the Dino-Lite should be an important instrument for restorers of art and other museum objects.
"The Dino-Lite proved to be very flexible"
"It was the only way to notice the changes easily"
"Visitors can easily watch our work"
Eric Vereijken is the owner and director of an advanced, 47-hectare tomato cultivation business. His enterprise is distributed across six locations in Brabant and Westland, two regions in the Netherlands that are of international significance for the ornamental plant and produce industries.
. Thanks to his many years of experience, Vereijken has optimised the cultivation of tomatoes in greenhouses. However, there was one factor that threatened that success until recently: the gall mite. It is a damaging pest that cannot be seen by the naked eye. However, the effects of the gall mite are plainly visible. Vereiken says "The mite consumes leaves and stems in the greenhouses. You only realise it once the leaves have begun to fade. By then, the gall mite has already been at work for a while". The tomato farmer noticed that, recently, this pest had appeared in his greenhouses more frequently and in greater numbers.
"The risk of realising too late that gall mites have been active continues to grow. If plants are harmed too much, they die and the damage is significant. First, we tried to find the gall mites with a magnifying glass, but they were just too small. After that, I tried putting them under a traditional microscope at the office. That allows you to see them. However, that means you have to pull samples from throughout the greenhouse, with the risk of new infections. The effect turned out to be very limited. It is difficult, if not impossible, to get a good idea of their spread using this method. Furthermore, this approach is extremely time-consuming and, ultimately, expensive as well".
Customised work for maximum effect
The business owner was at the end of his rope and consulted the experts at LTO Glaskracht, the Dutch trade association for greenhouse cultivation. "LTO Glaskracht brainstormed with us and that is how we found out about Dino-Lite. Dino-Lite Europe came to our business for a demonstration". And the rest is history. Eric Vereijken's teams have since been working with multiple models of these mobile microscopes, which have been adjusted precisely to their needs. He feels that he has finally got a handle on the problem. "My employees walk through the plants, determine whether they have brown stems or leaves, and mark them. These plants are treated for gall mites. The person responsible for disease control regularly checks a number of plants with the Dino-Lite. These send your smartphone or tablet a razor-sharp image of the leaf or stem on location, magnified up to 230 times. Since we can also create videos with the Dino-Lite, we can now see whether the mites are living or dead for the first time. That is important, because, after a localised spraying, we can see whether a treatment has had any effect".
Localised spraying is customised work. Spraying the entire greenhouse does not make any sense and is expensive. Furthermore, buyers in the sector do not appreciate pest control agents being used without careful thought. With the aid of the Dino-Lite, Vereijken's staff are able to find the exact locations where concentrated spraying will have the maximum effect.
Suitable for other plants as well
Eric says "The gall mite is a growing problem for the cultivation of tomatoes under grow lights. It is high time that for an effective tool that can be used to determine the level of infestation and required control efforts quickly and well". He expects that his fellow cultivators can benefit from handy, mobile, digital Dino-Lite microscopes, just like he did. Dino-Lite can make just as much of a difference for other plants with gall mites and different pests. Dino-Lite appears to be a solution for promptly detecting mites, lice, parasites, spores and other disease carriers in the flower industry as well.
Eric Vereijken says "In our experience, it works quickly and easily for the most part. Not to mention, you can film with this Dino-Lite tool. It is useful for convincingly showing others what is going on."
Razor-sharp photos of text details
Wil Fagel is a handwriting expert in Amsterdam. He performs forensic research and using his 35 years of experience and great precision, can determine whether handwriting belongs to a certain person. There is a stereo microscope on his desk for analysing the details of written texts. However, in addition to that traditional microscope, he often uses his digital microscope from Dino-Lite. "I always take this tool with me on location when I go to look at handwriting such as at a notary office. But the Dino-Lite is also perfect for taking razor-sharp digital photos in my own workroom of details that stand out to me in written text.
With his expertise, Wil Fagel is brought in for civil and criminal cases. As a handwriting expert, he is registered with the International Criminal Court in The Hague, he has previously worked for the International Criminal Tribunal for the former Yugoslavia, and he is active within The Netherlands Forensic Institute (NFI) as well as several other organisations. A colleague at this institute brought Dino-Lite to his attention. He selected the AM4113T-FVW model. "Using it, you view things under white light. Not that yellow lamplight, but daylight. That makes viewing better. What I also find useful is that you can switch between white light and UV light with this model. Depending on the wavelength, this allows you to see subtle differences in colour or, for instance, whether or not someone has just added a zero to the amount on a bill.
With his Dino-Lite, he brings elements to light on location that appear to escape the average viewer. How crossed lines in a letter or signature move exactly, the direction that a dot was written on a letter, the white "grinding traces" in a ballpoint pen line that indicate whether a loop rotates to the left or right: these can all contribute to identifying the author of the text being examined or the authenticity of a signature. What is real and what is fake? The answer to this question can make a significant financial difference. Fagel once examined the signature under a number of etchings that were attributed to the famous artist Anton Heyboer. "I could not make any judgement on the etchings themselves, but it was clear that the signatures on the etchings displayed differences when compared to the signature of the artist himself.
He continues to treasure the sturdy stereo microscope on his desk, but stresses that he can no longer do without his other mobile viewing tool. "Because I am so often off site for handwriting research, the Dino-Lite has become an indispensable part of my work."
See also: www.wffo.nl
"I find it useful that you can switch between white light and UV light"
"Dino-Lite is an indispensable part of off-site work"
Researching graphical quality at the Print Technology Division of the Warsaw University of Technology.
The Print Technology Division of the Warsaw University of Technology in Poland is using four types of Dino-Lite digital microscopes to analyse the quality of printed images and photographs. This research includes zooming in on microscopic changes in paper and card stock during the printing process. In addition, the university's researchers use the digital microscopes to monitor the various stages of book-binding. These include cutting, folding and testing the physical endurance of bound books.
"Excellent image analysis quality for a fair price"
Dr. Georgij Petriaszwili, professor at the Print Technology Division, says, "Dino-Lite provides excellent image analysis quality for a fair price. Until recently we were using other microscope brands. They were expensive and didn't always meet our needs. I first learned about the quality of Dino-Lite during a visit to the Arteveldehogeschool [Artevelde University College] in Ghent, Belgium. When I returned home to Poland, I started reading more about the options and the diverse models of Dino-Lite digital microscopes. I was pleasantly surprised. What's really important for us is that, in spite of the differences between Dino-Lite microscopes, the performance and support software form a well-coordinated package. We're definitely keeping track of any new developments from Dino-Lite. I expect that we'll be using the WiFi features in the near future to process greater numbers of images online."
The Print Technology Division of the Warsaw University of Technology trains specialists in analysing the image quality of graphical products. The division cooperates closely with graphics companies and printers throughout Poland. By 2018, the division will have been in existence for fifty years. Thus far, the division has trained over two thousand scientific professionals in the field of graphics arts. New generations of graphics researchers at the Warsaw University of Technology are using the following Dino-Lite microscopes: Edge AM7115 MZT, AM4113T-FVW, Premier AM3713 TB, Premier AM41132 TL (R4).
"A well-coordinated package"
Fluorescence Guided Surgery, FGS.
For surgeons it is of the utmost importance to be able to able to see accurately the tumour margins during the surgery. This is of particular importance for resection of metastatic diseases. An important and For FGS, clinically approved fluorescent dyes are being used to label the abnormal cells or diseased tissue. Illuminating the area of interest with the corresponding excitation light and the use of a correct emission filter in front of the imaging device, the cancerous tissue will light up and can be removed easily and accurately. With this method smaller tumours can be removed, opposed to the standard methods of visualisation and detection. FGS is nowadays considered as an effective way to carry out cancer surgery in comparison to the conventional surgery. Researchers around the world are exploring the possibilities of this emerging technology.
In the following 4 open access research papers, several different Dino-Lite Fluorescence microscopes have been used to perform Fluorescence Guided Surgery successfully.
FGS of Liver Metastasis in Orthotopic Nude-Mouse Models:The fluorescence Dino-Lite model AM4113T-GFBW has been used to visualize the green fluorescence protein (GFP) labelled tumour before, during and after the surgery.
The researchers mentioned below have performed a trial to show prove that FGS is an effective method for liver metastasis. Fourteen 14 mice of the created orthotopic liver metastasis model had been randomly divided in 2 groups. One group was treated with BLS (Bright Light Surgery) and the other group was treated with FGS by using the Dino-Lite fluorescence digital microscope. Post-surgical residual was clearly present with the BLS treated mice, while the FGS group showed no sign of residual tumor.
Paper: Murakami T, Hiroshima Y, Zhang Y, Chishima T, Tanaka K, Bouvet M, et al (2015) Fluorescence-Guided Surgery of Liver Metastasis in Orthotopic Nude-Mouse Models.
Link: PloS ONE 10(10): e0138752. Doi:10.1371/journal.pone.0138752
FGS using In Situ GFP Labeling with a Telomerase-Dependent Adenovirus in an Orthotopic Mouse Model
In this research article they demonstrate that labelling colon-cancer liver metastasis in-situ with the GFP OBP-401 adenovirus is a powerful tool to complete resection with FGS. Compared to the conventional BLS ( Bright Light Surgery) it reduced the recurrence rate and prolonged the over-all survival. FGS was performed under GFP guidance using amongst others the Dino-Lite fluorescence microscope AM4113T-GFBW.
Paper: Yano S, Takehara K, Miwa S, Kishimoto H, Hiroshima Y, Murakami T, et al. (2016) Improved Resection and Outcome of Colon-Cancer Liver Metastasis with Fluorescence-Guided Surgery Using In Situ GFP Labeling with a Telomerase-Dependent Adenovirus in an Orthotopic Mouse Model.
Link: PLoS ONE 11(2): e0148760. doi:10.1371/journal. pone.0148760
Color-coded FGS to Resect the Tumor along with the Tumor Microenvironment“Color coded FGS is an effective method to completely resect cancer cells along with the stromal cells in the TME which interact in a highly-complex pattern.“
Results: Using the Dino-Lite, subcutaneous tumors and the tumor microenvironment were clearly visualized and resected.” During this research they have used the AM4113T-YFGW to visualize the EL-4-RFP stained cells and the AM4113T-GFBW for the GFP stained cells.
Paper: Color-coded Imaging Enables Fluorescence-Guided Surgery to Resect the Tumor Along with the Tumor Microenvironment in a Syngeneic Mouse Model of EL-4 Lymphoma. Hasegawa K, Suetsugu A, Nakamura M, Matsumoto T, Kunisada T, Shimizu M, Saji S, Moriwaki H, Bouvet M, and Hoffman R.
Link: Anticancer research 36: 4443-4448 (2016). doi:10.21873/anticanres.10988
Color-coding cancer and stromal cells with genetic reporters enhances FGSAlso here GFP containing the OBP401 was used to label the cancer cells of the pancreatic cancer PDOX. The PDOX was previously grown in a RFP transgenic mouse. This dual color-coding enabled FGS to completely resect the pancreatic tumors including stroma. “Dual-colored FGS significantly prevented local recurrence, while bright-light surgery (BLS) or single color could not”. “The Dino-Lite mobile imaging system was used for imaging in live mice.” “This all-in-one compact digital camera makes the Dino-Lite imaging system easily transportable and thereby suitable for FGS”.
Paper: S Yano, Y Hiroshima, A Maawy, H Kishimoto, A Suetsugu, S Miwa, M Toneri, M Yamamoto, M H G Katz, J B Fleming, Y Urata, H Tazawa, S Kagawa, M Bouvet, T Fujiwara and R M Hoffman. Color-coding cancer and stromal cells with genetic reporters in a patient-derived orthotopic xenograft (PDOX) model of pancreatic cancer enhances fluorescence-guided surgery.
Link: Cancer Gene Therapy 22, 344-350 (July 2015) | doi:10.1038/cgt.2015.26
Dino-Lite indispensable for fieldwork
More and more scientists that do research in the field cannot function without their Dino-Lite digital microscope. An early user is Michele Hayeur Smith, an anthropological archaeologist at the Haffenreffer Museum of Anthropology at Brown University in Providence, Rhode Island, USA. She often travels to do her research in museums and at archaeological sites. Michèle is researching archaeological textiles from Norse societies of the North Atlantic, such as Iceland, and Greenland. "The Dino-Lite is especially ideal for my field research", she says.
Michèle is studying Viking Age and medieval textiles from Norse societies. Her research would not be possible without a Dino-Lite microscope: "The aim of my research is to understand the roles of women in Norse societies of the North Atlantic by looking at an activity exclusively carried out by women: textile production. In Iceland, for example, it has become very clear that textiles were used as a form of legal currency during the medieval period, when fish and wool were traded for goods that were not available in Iceland. In Greenland my analysis of textiles has shown that women were modifying traditional weaving techniques to adapt to the increasing cooling climates of the 14th century.
Michèle’s research has taken her far from home. She has visited the National Museum of Iceland, Greenland Museum and Archives, the National Museum of Denmark and the National Museum of Scotland, and the Bergen Museum. Both time and budget are issues with any scientific research project, thus, she is very happy with Dino-Lite considering the price of the instrument, its performance and the ease with which one can use the digital microscope: "I analyze the textile, both with and without the Dino-Lite. The Dino-Lite allows me to see microscopic detail of wools, silks or linens, to see how the fibers were spun, how they were woven and whether they were contaminated with insects or not. Usually I place the Dino-Lite directly on the textile fragment, and as a result rarely make use of the stand. The direct placement gives me maximum visibility, and I can use the maximum magnification so that I can study the structure of the fabric to the smallest detail. The images are stored on my computer linked to specific folders from the research sites. In Iceland I have already analyzed textile material with my Dino-Lite from 36 sites. All other information is incorporated into an Excel spreadsheet to help me find similarities and patterns in the way the fabric was made. This can tell us something about the trade in textiles, the use of raw materials and other important social issues."
The handy size of the Dino-Lite is convenient for Michèle’s research and travel requirements, "I just take it in my luggage. Do I have any suggestions for improvement? Yes, I would like to have one of the models that magnifies even more to see more detail!" Michèle’s enthusiasm has already encouraged the National Museum of Iceland and some of her students to purchase a Dino-Lite. "Given the options, it is a very affordable instrument. In addition, the microscope is handy and easy to use which is very pleasant considering the itinerant nature of my work. I can recommend Dino-Lite to every researcher needing microscopy in the field."