Eric Vereijken is the owner and director of an advanced, 47-hectare tomato cultivation business. His enterprise is distributed across six locations in Brabant and Westland, two regions in the Netherlands that are of international significance for the ornamental plant and produce industries.
. Thanks to his many years of experience, Vereijken has optimised the cultivation of tomatoes in greenhouses. However, there was one factor that threatened that success until recently: the gall mite. It is a damaging pest that cannot be seen by the naked eye. However, the effects of the gall mite are plainly visible. Vereiken says "The mite consumes leaves and stems in the greenhouses. You only realise it once the leaves have begun to fade. By then, the gall mite has already been at work for a while". The tomato farmer noticed that, recently, this pest had appeared in his greenhouses more frequently and in greater numbers.
"The risk of realising too late that gall mites have been active continues to grow. If plants are harmed too much, they die and the damage is significant. First, we tried to find the gall mites with a magnifying glass, but they were just too small. After that, I tried putting them under a traditional microscope at the office. That allows you to see them. However, that means you have to pull samples from throughout the greenhouse, with the risk of new infections. The effect turned out to be very limited. It is difficult, if not impossible, to get a good idea of their spread using this method. Furthermore, this approach is extremely time-consuming and, ultimately, expensive as well".
Customised work for maximum effect
The business owner was at the end of his rope and consulted the experts at LTO Glaskracht, the Dutch trade association for greenhouse cultivation. "LTO Glaskracht brainstormed with us and that is how we found out about Dino-Lite. Dino-Lite Europe came to our business for a demonstration". And the rest is history. Eric Vereijken's teams have since been working with multiple models of these mobile microscopes, which have been adjusted precisely to their needs. He feels that he has finally got a handle on the problem. "My employees walk through the plants, determine whether they have brown stems or leaves, and mark them. These plants are treated for gall mites. The person responsible for disease control regularly checks a number of plants with the Dino-Lite. These send your smartphone or tablet a razor-sharp image of the leaf or stem on location, magnified up to 230 times. Since we can also create videos with the Dino-Lite, we can now see whether the mites are living or dead for the first time. That is important, because, after a localised spraying, we can see whether a treatment has had any effect".
Localised spraying is customised work. Spraying the entire greenhouse does not make any sense and is expensive. Furthermore, buyers in the sector do not appreciate pest control agents being used without careful thought. With the aid of the Dino-Lite, Vereijken's staff are able to find the exact locations where concentrated spraying will have the maximum effect.
Suitable for other plants as well
Eric says "The gall mite is a growing problem for the cultivation of tomatoes under grow lights. It is high time that for an effective tool that can be used to determine the level of infestation and required control efforts quickly and well". He expects that his fellow cultivators can benefit from handy, mobile, digital Dino-Lite microscopes, just like he did. Dino-Lite can make just as much of a difference for other plants with gall mites and different pests. Dino-Lite appears to be a solution for promptly detecting mites, lice, parasites, spores and other disease carriers in the flower industry as well.
Eric Vereijken says "In our experience, it works quickly and easily for the most part. Not to mention, you can film with this Dino-Lite tool. It is useful for convincingly showing others what is going on."
"It works quickly and easily for the most part"
"Not to mention, you can even film with it"
Razor-sharp photos of text details
Wil Fagel is a handwriting expert in Amsterdam. He performs forensic research and using his 35 years of experience and great precision, can determine whether handwriting belongs to a certain person. There is a stereo microscope on his desk for analysing the details of written texts. However, in addition to that traditional microscope, he often uses his digital microscope from Dino-Lite. "I always take this tool with me on location when I go to look at handwriting such as at a notary office. But the Dino-Lite is also perfect for taking razor-sharp digital photos in my own workroom of details that stand out to me in written text.
With his expertise, Wil Fagel is brought in for civil and criminal cases. As a handwriting expert, he is registered with the International Criminal Court in The Hague, he has previously worked for the International Criminal Tribunal for the former Yugoslavia, and he is active within The Netherlands Forensic Institute (NFI) as well as several other organisations. A colleague at this institute brought Dino-Lite to his attention. He selected the AM4113T-FVW model. "Using it, you view things under white light. Not that yellow lamplight, but daylight. That makes viewing better. What I also find useful is that you can switch between white light and UV light with this model. Depending on the wavelength, this allows you to see subtle differences in colour or, for instance, whether or not someone has just added a zero to the amount on a bill.
With his Dino-Lite, he brings elements to light on location that appear to escape the average viewer. How crossed lines in a letter or signature move exactly, the direction that a dot was written on a letter, the white "grinding traces" in a ballpoint pen line that indicate whether a loop rotates to the left or right: these can all contribute to identifying the author of the text being examined or the authenticity of a signature. What is real and what is fake? The answer to this question can make a significant financial difference. Fagel once examined the signature under a number of etchings that were attributed to the famous artist Anton Heyboer. "I could not make any judgement on the etchings themselves, but it was clear that the signatures on the etchings displayed differences when compared to the signature of the artist himself.
He continues to treasure the sturdy stereo microscope on his desk, but stresses that he can no longer do without his other mobile viewing tool. "Because I am so often off site for handwriting research, the Dino-Lite has become an indispensable part of my work."
See also: www.wffo.nl
"I find it useful that you can switch between white light and UV light"
"Dino-Lite is an indispensable part of off-site work"
Researching graphical quality at the Print Technology Division of the Warsaw University of Technology.
The Print Technology Division of the Warsaw University of Technology in Poland is using four types of Dino-Lite digital microscopes to analyse the quality of printed images and photographs. This research includes zooming in on microscopic changes in paper and card stock during the printing process. In addition, the university's researchers use the digital microscopes to monitor the various stages of book-binding. These include cutting, folding and testing the physical endurance of bound books.
"Excellent image analysis quality for a fair price"
Dr. Georgij Petriaszwili, professor at the Print Technology Division, says, "Dino-Lite provides excellent image analysis quality for a fair price. Until recently we were using other microscope brands. They were expensive and didn't always meet our needs. I first learned about the quality of Dino-Lite during a visit to the Arteveldehogeschool [Artevelde University College] in Ghent, Belgium. When I returned home to Poland, I started reading more about the options and the diverse models of Dino-Lite digital microscopes. I was pleasantly surprised. What's really important for us is that, in spite of the differences between Dino-Lite microscopes, the performance and support software form a well-coordinated package. We're definitely keeping track of any new developments from Dino-Lite. I expect that we'll be using the WiFi features in the near future to process greater numbers of images online."
The Print Technology Division of the Warsaw University of Technology trains specialists in analysing the image quality of graphical products. The division cooperates closely with graphics companies and printers throughout Poland. By 2018, the division will have been in existence for fifty years. Thus far, the division has trained over two thousand scientific professionals in the field of graphics arts. New generations of graphics researchers at the Warsaw University of Technology are using the following Dino-Lite microscopes: Edge AM7115 MZT, AM4113T-FVW, Premier AM3713 TB, Premier AM41132 TL (R4).
"A well-coordinated package"
Fluorescence Guided Surgery, FGS.
For surgeons it is of the utmost importance to be able to able to see accurately the tumour margins during the surgery. This is of particular importance for resection of metastatic diseases. An important and For FGS, clinically approved fluorescent dyes are being used to label the abnormal cells or diseased tissue. Illuminating the area of interest with the corresponding excitation light and the use of a correct emission filter in front of the imaging device, the cancerous tissue will light up and can be removed easily and accurately. With this method smaller tumours can be removed, opposed to the standard methods of visualisation and detection. FGS is nowadays considered as an effective way to carry out cancer surgery in comparison to the conventional surgery. Researchers around the world are exploring the possibilities of this emerging technology.
In the following 4 open access research papers, several different Dino-Lite Fluorescence microscopes have been used to perform Fluorescence Guided Surgery successfully.
FGS of Liver Metastasis in Orthotopic Nude-Mouse Models:The fluorescence Dino-Lite model AM4113T-GFBW has been used to visualize the green fluorescence protein (GFP) labelled tumour before, during and after the surgery.
The researchers mentioned below have performed a trial to show prove that FGS is an effective method for liver metastasis. Fourteen 14 mice of the created orthotopic liver metastasis model had been randomly divided in 2 groups. One group was treated with BLS (Bright Light Surgery) and the other group was treated with FGS by using the Dino-Lite fluorescence digital microscope. Post-surgical residual was clearly present with the BLS treated mice, while the FGS group showed no sign of residual tumor.
Paper: Murakami T, Hiroshima Y, Zhang Y, Chishima T, Tanaka K, Bouvet M, et al (2015) Fluorescence-Guided Surgery of Liver Metastasis in Orthotopic Nude-Mouse Models.
Link: PloS ONE 10(10): e0138752. Doi:10.1371/journal.pone.0138752
FGS using In Situ GFP Labeling with a Telomerase-Dependent Adenovirus in an Orthotopic Mouse Model
In this research article they demonstrate that labelling colon-cancer liver metastasis in-situ with the GFP OBP-401 adenovirus is a powerful tool to complete resection with FGS. Compared to the conventional BLS ( Bright Light Surgery) it reduced the recurrence rate and prolonged the over-all survival. FGS was performed under GFP guidance using amongst others the Dino-Lite fluorescence microscope AM4113T-GFBW.
Paper: Yano S, Takehara K, Miwa S, Kishimoto H, Hiroshima Y, Murakami T, et al. (2016) Improved Resection and Outcome of Colon-Cancer Liver Metastasis with Fluorescence-Guided Surgery Using In Situ GFP Labeling with a Telomerase-Dependent Adenovirus in an Orthotopic Mouse Model.
Link: PLoS ONE 11(2): e0148760. doi:10.1371/journal. pone.0148760
Color-coded FGS to Resect the Tumor along with the Tumor Microenvironment“Color coded FGS is an effective method to completely resect cancer cells along with the stromal cells in the TME which interact in a highly-complex pattern.“
Results: Using the Dino-Lite, subcutaneous tumors and the tumor microenvironment were clearly visualized and resected.” During this research they have used the AM4113T-YFGW to visualize the EL-4-RFP stained cells and the AM4113T-GFBW for the GFP stained cells.
Paper: Color-coded Imaging Enables Fluorescence-Guided Surgery to Resect the Tumor Along with the Tumor Microenvironment in a Syngeneic Mouse Model of EL-4 Lymphoma. Hasegawa K, Suetsugu A, Nakamura M, Matsumoto T, Kunisada T, Shimizu M, Saji S, Moriwaki H, Bouvet M, and Hoffman R.
Link: Anticancer research 36: 4443-4448 (2016). doi:10.21873/anticanres.10988
Color-coding cancer and stromal cells with genetic reporters enhances FGSAlso here GFP containing the OBP401 was used to label the cancer cells of the pancreatic cancer PDOX. The PDOX was previously grown in a RFP transgenic mouse. This dual color-coding enabled FGS to completely resect the pancreatic tumors including stroma. “Dual-colored FGS significantly prevented local recurrence, while bright-light surgery (BLS) or single color could not”. “The Dino-Lite mobile imaging system was used for imaging in live mice.” “This all-in-one compact digital camera makes the Dino-Lite imaging system easily transportable and thereby suitable for FGS”.
Paper: S Yano, Y Hiroshima, A Maawy, H Kishimoto, A Suetsugu, S Miwa, M Toneri, M Yamamoto, M H G Katz, J B Fleming, Y Urata, H Tazawa, S Kagawa, M Bouvet, T Fujiwara and R M Hoffman. Color-coding cancer and stromal cells with genetic reporters in a patient-derived orthotopic xenograft (PDOX) model of pancreatic cancer enhances fluorescence-guided surgery.
Link: Cancer Gene Therapy 22, 344-350 (July 2015) | doi:10.1038/cgt.2015.26
Dino-Lite indispensable for fieldwork
More and more scientists that do research in the field cannot function without their Dino-Lite digital microscope. An early user is Michele Hayeur Smith, an anthropological archaeologist at the Haffenreffer Museum of Anthropology at Brown University in Providence, Rhode Island, USA. She often travels to do her research in museums and at archaeological sites. Michèle is researching archaeological textiles from Norse societies of the North Atlantic, such as Iceland, and Greenland. "The Dino-Lite is especially ideal for my field research", she says.
Michèle is studying Viking Age and medieval textiles from Norse societies. Her research would not be possible without a Dino-Lite microscope: "The aim of my research is to understand the roles of women in Norse societies of the North Atlantic by looking at an activity exclusively carried out by women: textile production. In Iceland, for example, it has become very clear that textiles were used as a form of legal currency during the medieval period, when fish and wool were traded for goods that were not available in Iceland. In Greenland my analysis of textiles has shown that women were modifying traditional weaving techniques to adapt to the increasing cooling climates of the 14th century.
Michèle’s research has taken her far from home. She has visited the National Museum of Iceland, Greenland Museum and Archives, the National Museum of Denmark and the National Museum of Scotland, and the Bergen Museum. Both time and budget are issues with any scientific research project, thus, she is very happy with Dino-Lite considering the price of the instrument, its performance and the ease with which one can use the digital microscope: "I analyze the textile, both with and without the Dino-Lite. The Dino-Lite allows me to see microscopic detail of wools, silks or linens, to see how the fibers were spun, how they were woven and whether they were contaminated with insects or not. Usually I place the Dino-Lite directly on the textile fragment, and as a result rarely make use of the stand. The direct placement gives me maximum visibility, and I can use the maximum magnification so that I can study the structure of the fabric to the smallest detail. The images are stored on my computer linked to specific folders from the research sites. In Iceland I have already analyzed textile material with my Dino-Lite from 36 sites. All other information is incorporated into an Excel spreadsheet to help me find similarities and patterns in the way the fabric was made. This can tell us something about the trade in textiles, the use of raw materials and other important social issues."
The handy size of the Dino-Lite is convenient for Michèle’s research and travel requirements, "I just take it in my luggage. Do I have any suggestions for improvement? Yes, I would like to have one of the models that magnifies even more to see more detail!" Michèle’s enthusiasm has already encouraged the National Museum of Iceland and some of her students to purchase a Dino-Lite. "Given the options, it is a very affordable instrument. In addition, the microscope is handy and easy to use which is very pleasant considering the itinerant nature of my work. I can recommend Dino-Lite to every researcher needing microscopy in the field."
No cap without Dino-Lite
You may not be aware of it, but behind each cap you turn lies a hidden piece of science. Caps should not only seal perfectly, they must also be easily machined and each consumer should be able to open them without problems. The Swiss Corvaglia Group knows all about these issues.
The Corvaglia group based in Eschlikon in Switzerland consists of Corvaglia Mould AG and Corvaglia Closures AG. Corvaglia Mould AG develops, designs and tests the injection molds that Corvaglia Closures AG, amongst others, uses to produce closures for all kinds of beverages in so-called PET bottles. Carsten Peil is Head of Research Engineering at Corvaglia Mould AG: "Approximately 20 percent of all PET bottle caps in the world are made with injection molds made by us. Corvaglia Closures AG supplies fasteners to all major brands for a wide range of products, from soft drinks with and without carbonic to juices. Fruit juices are often bottled aseptically which means that our caps must also be aseptic. That poses demands on the design of the caps that must be easy to remove despite the seal. As you can see, the design, the making of the injection molds and manufacturing the caps forms a true science. The making of the molds is one of the areas where we use the Dino-Lite USB microscopes."
The weight of 0.05 grams
Making the injection molds is a labor intensive process which operates with an accuracy of many decimal places. Many molds are made in low-wage countries, but Corvaglia Mould AG makes everything in-house. Peil: "We have the experience that nobody else has, and only we have the machinery and professionals in house to achieve a perfect finished product. We know exactly how high-density polyethylene and polypropylene behave, the two substances that the closures are made off. "Even for Corvaglia Mould AG it is a challenge every time to create a closure that meets all customer requirements. "You always try to make the lightest possible closure to reduce costs. Annually approximately 400 billion beverage closures are used worldwide. Working with such numbers, achieving a slight reduction of 0,05 grams has a huge economic and ecological impact."
Both in making and assessing the injection molds and the first audit of the products from the molds, Dino-Lite microscopes are used by Corvaglia Mould AG. Peil: "Since many of our people should have access to a good microscope, we have chosen to work with a number of Dino-Lites because they are versatile and flexible, the quality of the pictures is good just like the degree of magnification and the built-in LED lighting. Of course you can choose to buy one expensive high-end machine, but with using several Dino-Lites we avoid a lot of walking back-and-forth and waiting time. Now anyone can use a Dino-Lite with their own PC. They are fully integrated into our work process, to our complete satisfaction."
Dino-Lite TrichoScope increases turnover for hairdressers and beauty parlours
The Italian company Exagon Engineering is leading in the development of software for retail organisations, especially hairdressers and beauty parlours. The company has developed software that in combination with the Dino-Lite TrichoScope will help to find the right products to treat hair and skin problems. The number of products for hair treatment and improvement in a hair salon is huge. This is also true for the sheer amount of different cosmetics products in a beauty parlour. Selling these products is of high value to the hairdresser or cosmetician, but in practice this type of upsell in their stores is not easy. Their customer are in doubt because of lack of evidence about the usefulness.
To address this problem Exagon developed software that can analyse possible skin and hair problems by using a Dino-Lite TrichoScope, a digital microscope. Exagon director Antonio Visconti: “Trichoscopy is the young science studying the health of hair and scalp. The customer can see the pictures while the hairdresser or cosmetician is doing the examination. With the strongly magnified image of hair, scalp or skin the Exagon Engineering software creates an advice about the product or products that the hairdresser or cosmetician can recommend. Using the Dino-Lite TrichoScope with the Exagon software increases the total sale of hair and skin treatment products strongly.”
Easy software integration
Exagon Engineering is able to customize the software for every hairdresser, beauty parlour supplier of cosmetic products, assuring that only products are recommended that are actually available. Visconti: “At the moment we have the details of more than 300 manufacturers in our database, allowing us to create a tailor made solution for each customer. The Dino-Lite TrichoScope has proven to be the best product for this application. The scalp, follicles and hair can be studied in close detail. Thanks to the Software Development Kit (SDK) supplied by Dino-Lite the integration with our software has become very simple, which was a major reason to choose Dino-Lite.”
The TrichoScope is sturdy, it allows for magnifications up 200 and even 500 times with resolutions of 1,3 or 5 megapixel, depending on the model. The TrichoScope is CE certified as a medical device. It has 8 integrated LED making an external light source unnecessary and with power coming from the USB connection there is no need to replace or recharge batteries. These properties make the Dino-Lite TrichoScope the right choice for every hairdresser, beauty parlour or manufacturer of cosmetics products.